{"id":181,"date":"2025-02-27T09:23:04","date_gmt":"2025-02-27T09:23:04","guid":{"rendered":"https:\/\/imb.am\/scf\/?page_id=181"},"modified":"2026-03-20T09:10:19","modified_gmt":"2026-03-20T09:10:19","slug":"submit-samples","status":"publish","type":"page","link":"https:\/\/imb.am\/scf\/submit-samples\/","title":{"rendered":"SUBMIT SAMPLES\u00a0\u00a0\u00a0\u00a0"},"content":{"rendered":"\t\t
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To ensure high-quality processing and sequencing, please follow the instructions below for sample preparation, quality requirements, and shipping procedures.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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General Shipping Instructions<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Packaging<\/em>: Use leak-proof, insulated containers with secure closures. Seal sample tubes with parafilm to prevent leakage or evaporation.
Tube Labeling<\/em>: Clearly label each tube with a unique sample ID that matches the sample submission form. Use alcohol-resistant\/permanent markers to prevent smudging during handling and disinfection.<\/p>


Shipping Conditions:<\/em>
Libraries:<\/strong> Ship on dry ice
DNA:<\/strong> Ship on dry ice or cold packs (dry ice preferred)
RNA<\/strong>: Ship on dry ice only
Blood\/Cells<\/strong>: See specific conditions in the sample type sections below
Sample Submission Form:<\/strong> Include a printed and emailed version. Ensure the sample names exactly match those on the tubes.
Shipping Address:<\/strong> 7 Ezras Hasratyan St., 0014, Institute of Molecular Biology NAS RA, Yerevan, Armenia.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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Sample Preparation and Sequencing Support<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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The core facility offers comprehensive services, not only sequencing with user-supplied libraries but also full project support, including library preparation according to standard protocols for whole exome sequencing<\/strong>, reduced representation bisulfite sequencing<\/strong>, and transcriptome sequencing<\/strong>. We are open to discussing other\/custom protocols and applications.\u00a0\u00a0<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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User-Supplied Libraries<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Format<\/em>: Submit in 1.5 mL low-bind microcentrifuge tubes
Concentration<\/em>: \u2265 30 nM
Volume<\/em>: Minimum 10\u201320 \u03bcL per library
Buffer<\/em>: 10 mM Tris-HCl, pH 8.0 (no EDTA or salts) or RSB
Pooling:<\/em> If libraries are already pooled, indicate the pooling scheme and desired loading concentration.
QC Requirements<\/em>: Provide TapeStation traces and Qubit quantification.
Labeling<\/em>: Clearly label each tube with the sample name and concentration.
Shipping<\/em>: Ship on dry ice and include a detailed sample sheet with library names, barcodes, and pooling instructions if applicable.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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Sample Preparation and Sequencing Support<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Purity<\/em>: A260\/A280: 1.8-2.0, A260\/A230: 2.0-2.2.
Concentration<\/em>: 20\u201350 ng\/\u03bcL
Volume<\/em>: \u2265 50 \u03bcL
Amount<\/em>: \u2265 1\u20132 \u03bcg total DNA
Buffer<\/em>: Nuclease-free water or low-EDTA TE<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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RNA Sample Requirements<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Purity<\/em>: A260\/A280: ~2.0, A260\/A230: >1.8.
Integrity<\/em>: RIN \u2265 7 (Bioanalyzer\/TapeStation)
Concentration<\/em>: 5-20 ng\/\u03bcL
Volume<\/em>: \u2265 50 \u03bcL
Amount<\/em>: \u2265 1 \u03bcg total RNA
Buffer<\/em>: Nuclease-free water (no EDTA, salts, or inhibitors)<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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Blood and Blood Cell Samples<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Whole Blood<\/u>
For DNA extraction:<\/em>
Volume: 2\u20135 mL in EDTA tube
Ship at 4\u00b0C (cold packs), deliver within 24\u201348 hrs
For RNA extraction:<\/em>
Ship on dry ice
Store at \u221280\u00b0C if not shipping immediately<\/p>

PBMCs or Isolated Blood Cells<\/u>
Provide snap-frozen cell pellets (\u221280\u00b0C)
Cell count: 2\u20135 million cells per sample
Ship on dry ice
Indicate cell type and count on the form<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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Animal Cell Culture Samples<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Sample type:<\/em> Frozen cell pellets from cultured animal cells
Storage<\/em>: Snap-freeze in liquid nitrogen and store at \u221280\u00b0C
Amount<\/em>: 2\u20135 million cells per sample
Buffer<\/em>: Send as dry pellets
Shipping: <\/em>Ship on dry ice
Labeling<\/em>: Include cell line name, treatment condition (if applicable), and passage number (if known)<\/p>


Additional Notes<\/em>
All samples undergo internal quality control before processing. If samples do not meet quality or quantity requirements, we will contact you to discuss options (e.g., additional cleanup, resubmission, or extra charges). For special projects (e.g., degraded RNA, ultra-low input), please consult with us.<\/strong><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"

To ensure high-quality processing and sequencing, please follow the instructions below for sample preparation, quality requirements, and shipping procedures. General Shipping Instructions Packaging: Use leak-proof, insulated containers with secure closures. Seal sample tubes with parafilm to prevent leakage or evaporation.Tube Labeling: Clearly label each tube with a unique sample ID that matches the sample submission […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-181","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/imb.am\/scf\/wp-json\/wp\/v2\/pages\/181","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/imb.am\/scf\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/imb.am\/scf\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/imb.am\/scf\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/imb.am\/scf\/wp-json\/wp\/v2\/comments?post=181"}],"version-history":[{"count":43,"href":"https:\/\/imb.am\/scf\/wp-json\/wp\/v2\/pages\/181\/revisions"}],"predecessor-version":[{"id":677,"href":"https:\/\/imb.am\/scf\/wp-json\/wp\/v2\/pages\/181\/revisions\/677"}],"wp:attachment":[{"href":"https:\/\/imb.am\/scf\/wp-json\/wp\/v2\/media?parent=181"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}