{"id":161,"date":"2025-02-27T09:20:53","date_gmt":"2025-02-27T09:20:53","guid":{"rendered":"https:\/\/imb.am\/scf\/?page_id=161"},"modified":"2025-04-30T12:37:36","modified_gmt":"2025-04-30T12:37:36","slug":"instruments","status":"publish","type":"page","link":"https:\/\/imb.am\/scf\/instruments\/","title":{"rendered":"INSTRUMENTS"},"content":{"rendered":"\t\t
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Illumina NextSeq 2000<\/a><\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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\n\t\t\t\t\t\t\t\t\tA high-performance sequencer, offering scalability and flexibility for a wide variety of sequencing applications. With its ability to deliver up to 1.2 billion reads per run, the NextSeq 2000 provides cost-effective, high-throughput sequencing for large-scale projects.\n\nTo help plan experiments, please consult this table of flow-cell capacities.\u00a0 Note that each cluster can generate one single read or one read pair.\u00a0 All flow cells can be used in single-read or paired-end mode.\n\nAvailable flow cell size and cycle combinations.\n\n\n\n\n\n\n\n
Flowcell<\/strong><\/td>\n\nClusters<\/strong><\/td>\n50 cycles<\/strong><\/td>\n100<\/strong><\/td>\n200<\/strong><\/td>\n300<\/strong><\/td>\n600<\/strong><\/td>\n<\/tr>\n
P1<\/strong><\/td>\n\n100M<\/td>\n–<\/td>\nyes<\/td>\n–<\/td>\nyes<\/td>\nyes<\/td>\n<\/tr>\n
P2<\/strong><\/td>\n\n400M<\/td>\n–<\/td>\nyes<\/td>\nyes<\/td>\nyes<\/td>\nyes<\/td>\n<\/tr>\n
P3<\/strong><\/td>\n\n1200M<\/td>\n–<\/td>\nyes<\/td>\nyes<\/td>\nyes<\/td>\n–<\/td>\n<\/tr>\n
P4<\/strong><\/td>\n\n1600M<\/td>\nyes<\/td>\nyes<\/td>\nyes<\/td>\nyes<\/td>\n–<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\nApproximate number of samples that can be sequenced on each flow cell using the specified MR or MRP count.\n\n\n\n\n\n\n\n\n
Library<\/strong><\/td>\nMR(P)<\/strong><\/td>\nCycles<\/strong><\/td>\nP1<\/strong><\/td>\nP2<\/strong><\/td>\nP3<\/strong><\/td>\nP4<\/strong><\/td>\n<\/tr>\n
Small WG<\/td>\n<\/td>\n<\/td>\n7<\/td>\n32<\/td>\n92<\/td>\n138<\/td>\n<\/tr>\n
RNA-Seq mRNA<\/td>\n25<\/td>\n100(PE)<\/td>\n4<\/u><\/td>\n16<\/td>\n48<\/td>\n72<\/td>\n<\/tr>\n
RNA-Seq total<\/td>\n50<\/td>\n100(PE)<\/td>\n2<\/u><\/td>\n8<\/td>\n24<\/td>\n36<\/td>\n<\/tr>\n
RRBS<\/td>\n30<\/td>\n50,100PE<\/td>\n3<\/u><\/td>\n13<\/td>\n40<\/td>\n60<\/td>\n<\/tr>\n
WES<\/td>\n50<\/td>\n100PE<\/td>\n2<\/u><\/td>\n10<\/td>\n30<\/td>\n45<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t
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MinION (Nanopore) <\/a><\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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A portable sequencing device enabling real-time, long-read sequencing. With MinION, we provide unparalleled flexibility for smaller projects, especially those requiring real-time read filtering or long-read sequencing for structural variant detection, isoform resolution, or de novo assembly.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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Promega Maxwell RSC<\/a><\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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The Maxwell RSC Instrument is a compact, automated nucleic acid purification platform that processes up to 16 samples simultaneously. Using prefilled cartridges and preprogrammed methods, the Maxwell RSC offers consistent, reliable DNA or RNA extraction in 25\u201360 minutes, depending upon sample type.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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Agilent TapeStation 4150<\/a><\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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A critical tool for sample quality control (QC). The TapeStation system ensures precise and reproducible DNA, RNA, and protein analysis, providing high-resolution electrophoretic profiles for assessing sample integrity before sequencing.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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Qubit 4.0 (ThermoFisher Scientific)<\/a><\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Fast and sensitive quantification of DNA, RNA and proteins. The Invitrogen Qubit 4 Fluorometer is a benchtop fluorometer designed to accurately measure DNA, RNA, and protein concentration.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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Eppendorf Mastercycler nexus gradient<\/a><\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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The versatile PCR thermal cycler offers gradient functionality, allowing for the optimization of annealing temperatures in a single run. The device ensures reliable and consistent PCR results and is suitable for a wide range of applications in molecular biology.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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Biorad CFX-96<\/a><\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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A powerful real-time PCR instrument, the CFX-96 offers accurate and rapid quantification of nucleic acids, making it an essential component of QC and validation workflows.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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Illumina NextSeq 2000 A high-performance sequencer, offering scalability and flexibility for a wide variety of sequencing applications. With its ability to deliver up to 1.2 billion reads per run, the NextSeq 2000 provides cost-effective, high-throughput sequencing for large-scale projects. To help plan experiments, please consult this table of flow-cell capacities.\u00a0 Note that each cluster can […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-161","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/imb.am\/scf\/wp-json\/wp\/v2\/pages\/161","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/imb.am\/scf\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/imb.am\/scf\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/imb.am\/scf\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/imb.am\/scf\/wp-json\/wp\/v2\/comments?post=161"}],"version-history":[{"count":76,"href":"https:\/\/imb.am\/scf\/wp-json\/wp\/v2\/pages\/161\/revisions"}],"predecessor-version":[{"id":589,"href":"https:\/\/imb.am\/scf\/wp-json\/wp\/v2\/pages\/161\/revisions\/589"}],"wp:attachment":[{"href":"https:\/\/imb.am\/scf\/wp-json\/wp\/v2\/media?parent=161"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}